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                          Rabbit Anti-Bcl-2/Biotin Conjugated antibody (bs-0032R-Bio)
                          说 明 书: 100ul  
                          产品编号 bs-0032R-Bio
                          英文名称 Anti-Bcl-2/Biotin
                          中文名称 生物素标记的Bcl-2抗体
                          别    名 Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN.  
                          规格价格 100ul/2980元 购买        大包装/询价
                          说 明 书 100ul  
                          研究领域 细胞生物  信号转导  细胞凋亡  细胞类型标志物  肿瘤细胞生物标志物  新陈代谢  线粒体  
                          抗体来源 Rabbit
                          克隆类型 Polyclonal
                          交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, 
                          产品应用 WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200 
                          not yet tested in other applications.
                          optimal dilutions/concentrations should be determined by the end user.
                          分 子 量 26kDa
                          性    状 Lyophilized or Liquid
                          浓    度 1mg/ml
                          免 疫 原 KLH conjugated synthetic peptide derived from human Bcl-2
                          亚    型 IgG
                          纯化方法 affinity purified by Protein A
                          储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
                          保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
                          产品介绍 background:
                          The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18)-bearing follicular B cell lymphomas(1,2).Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5).Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7).A more recently identified protein,designated Bax p21(i.e., Bcl-associated X protein ),has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2(8). Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2(8).

                          Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

                          Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity. Interacts with EI24 (By similarity). Also interacts with APAF1, BBC3, BCL2L1, BNIPL, MRPL41 and TP53BP2. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets. Interacts with BAG1 in an ATP-dependent manner. Interacts with RAF1 (the 'Ser-338' and 'Ser-339' phosphorylated form). Interacts (via the BH4 domain) with EGLN3; the interaction prevents the formation of the BAX-BCL2 complex and inhibits the anti-apoptotic activity of BCL2. Interacts with G0S2; this interaction also prevents the formation of the anti-apoptotic BAX-BCL2 complex.

                          Subcellular Location:
                          Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.

                          Tissue Specificity:
                          Expressed in a variety of tissues.

                          Post-translational modifications:
                          Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
                          Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
                          Monoubiquitinated by PARK2, leading to increase its stability.

                          Note=A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.

                          Belongs to the Bcl-2 family.

                          Database links:

                          Entrez Gene: 281020 Cow

                          Entrez Gene: 596 Human

                          Entrez Gene: 12043 Mouse

                          Entrez Gene: 24224 Rat

                          Omim: 151430 Human

                          SwissProt: O02718 Cow

                          SwissProt: P10415 Human

                          SwissProt: P10417 Mouse

                          SwissProt: P49950 Rat

                          Unigene: 150749 Human

                          Unigene: 257460 Mouse

                          Unigene: 9996 Rat

                          Important Note:
                          This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

                          Bcl-2基因是指B-cell lymphoma gene。人体滤泡B细胞淋巴瘤中过量表达的原癌基因。由于染色体t(14;18)易位,将Bcl-2基因置于免疫球蛋白重链的转录调控下,使其表达失控。在细胞系中其过量表达能延长细胞存活期而不诱导细胞增殖。它是哺乳动物中细胞调亡的抑制基因。参与细胞凋亡的调控。肿瘤中的Bcl-2基因可提高侵润性瘤细胞的生存能力。主要用于滤胞型淋巴瘤、毛细管性白血病及细胞凋亡等方面的研究。

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