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                          Rabbit Anti-CD90 antibody (bs-0778R)
                          說明書: 50ul  100ul  200ul

                          產品編號 bs-0778R
                          英文名稱 CD90
                          中文名稱 CD90抗體
                          別    名 CD90 / Thy1; CD7; CD90; CD90 antigen; CDw90; FLJ33325; MGC128895; T25; Theta antigen; Thy-1; Thy 1; Thy 1 cell surface antigen; Thy 1 membrane glycoprotein; Thy 1 membrane glycoprotein precursor; Thy 1.2; Thy-1 T-cell antigen; Thy1 antigen; Thy1 T cell antigen; Thy1.1; Thy1.2; Thymus cell antigen 1, theta; THY1_RAT; THY1_HUMAN.  
                          Specific References  (12)     |     bs-0778R has been referenced in 12 publications.
                          [IF=2.94] Wang, Kai, et al. "Over-expression of Mash1 improves the GABAergic differentiation of bone marrow mesenchymal stem cells< i> in vitro." Brain Research Bulletin (2013).  FCM ;  Rat.  
                          [IF=2.88] Long, Qianfa, et al. "Genetically engineered bone marrow mesenchymal stem cells improve functional outcome in a rat model of epilepsy." Brain Research (2013).  Rat.  
                          [IF=2.26] Yu, Xian-huan, et al. "Clinicopathological characteristics of 20 cases of hepatocellular carcinoma with bile duct tumor thrombi." Digestive diseases and sciences 56.1 (2011): 252-259.  IHC-P ;  Human.  
                          [IF=6.72] Chen, Guobao, et al. "3D Scaffolds with Different Stiffness but Same Microstructure for Bone Tissue Engineering." ACS Applied Materials & Interfaces (2015).  IHC-F ;  Rat.  
                          [IF=1.52] Tepekoy, Filiz, et al. "CD90 and CD105 expression in the mouse ovary and testis at different stages of postnatal development." Reproductive Biology(2015).  IHC-P ;  Mouse.  
                          [IF=3.36] Long, Q., et al. "Bone marrow mesenchymal stem cell transplantation improves cognitive impairment via up-regulation of hippocampal GABAergic system in a rat model of chronic cerebral hypoperfusion." Neuroscience (2015).  Rat.  
                          [IF=1.80] Vansandt, Lindsey M., et al. "Conservation of spermatogonial stem cell marker expression in undifferentiated felid spermatogonia." Theriogenology(2016).  IHC-P ;  Other Species.  
                          [IF=1.43] Zeng, Biao, et al. "Increased expression of importin13 in endometriosis and endometrial carcinoma." Medical Science Monitor 18.6 (2012): CR361-CR367.  IHC-P ;  Human.  
                          [IF=1.06] Scott, Erin M., et al. "Early histopathologic changes in the retina and optic nerve in canine primary angle‐closure glaucoma." Veterinary ophthalmology16.s1 (2013): 79-86.  IHC-P ;  Dog.  
                          [IF=0.00] Gstraunthaler, Gerhard, et al. "Human platelet lysates successfully replace fetal bovine serum in adipose-derived adult stem cell culture." Journal of Advanced Biotechnology and Bioengineering 2.1 (2014): 1-11.  IF(ICC) ;  Human.  
                          [IF=0.52] Utomo, Pamudji, et al. "Decreasing SDF1-CXCR4 Expression after Adipose-Derived Mesenchymal Stem Cells (ASCS) Treatment Combined with Freeze-Dried Amniotic Membrane Wrapping in Rat Sciatic Nerve Injury." International Journal of ChemTech Research.  IF(ICC) ;  Rat.  
                          [IF=1.92] Ma, Caiyun, et al. "Cryopreservation and multipotential characteristics evaluation of a novel type of mesenchymal stem cells derived from Small Tailed Han Sheep fetal lung tissue." Cryobiology (2017).  FCM ;  Sheep.  
                          規格價格 50ul/780元 購買    100ul/1380元 購買    200ul/2200元 購買    大包裝/詢價
                          說 明 書 50ul  100ul  200ul
                          研究領域 細胞生物  免疫學  神經生物學  
                          抗體來源 Rabbit
                          克隆類型 Polyclonal
                          交叉反應 Human, Mouse, Rat, Dog, Pig, Horse, Sheep, 
                          產品應用 WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 (石蠟切片需做抗原修復)
                          not yet tested in other applications.
                          optimal dilutions/concentrations should be determined by the end user.
                          分 子 量 12kDa
                          細胞定位 細胞膜 
                          性    狀 Lyophilized or Liquid
                          濃    度 1mg/ml
                          免 疫 原 KLH conjugated synthetic peptide derived from rat Thy-1:31-120/161 <Extracellular>
                          亞    型 IgG
                          純化方法 affinity purified by Protein A
                          儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
                          保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
                          PubMed PubMed
                          產品介紹 background:
                          Thy-1 or CD90 (Cluster of Differentiation 90) is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.

                          May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

                          Subcellular Location:
                          Cell membrane; Lipid-anchor, GPI-anchor.

                          Tissue Specificity:
                          Abundant in lymphoid tissues.

                          Post-translational modifications:
                          Glycosylation is tissue specific. Sialylation of N-glycans at Asn-93 in brain and at Asn-42, Asn-93 and Asn-117 in thymus.

                          Contains 1 Ig-like V-type (immunoglobulin-like) domain.


                          Gene ID:

                          Database links:

                          Entrez Gene: 7070 Human

                          Entrez Gene: 21838 Mouse

                          Entrez Gene: 24832 Rat

                          Omim: 188230 Human

                          SwissProt: P04216 Human

                          SwissProt: P01831 Mouse

                          SwissProt: P01830 Rat

                          Unigene: 644697 Human

                          Unigene: 3951 Mouse

                          Unigene: 108198 Rat

                          Important Note:
                          This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

                          Brain (Mouse) Lysate at 40 ug
                          Primary: Anti-CD90 (bs-0778R) at 1/300 dilution
                          Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
                          Predicted band size: 12 kD
                          Observed band size: 32 kD
                          Brain (Mouse) Lysate at 40 ug
                          Primary: Anti-CD90 (bs-0778R) at 1/300 dilution
                          Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
                          Predicted band size: 12 kD
                          Observed band size: 32 kD
                          Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
                          Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
                          Incubation: Anti-Thy-1/CD90/ Thy1.1 Polyclonal Antibody, Unconjugated(bs-0778R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
                          Blank control: U937(blue).
                          Primary Antibody: Rabbit Anti-CD90 antibody(bs-0778R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
                          Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
                          Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
                          The cells were fixed with 2% paraformaldehyde (10 min).Primary antibody (bs-0778R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
                          Blank control (blue line): U251 (blue).
                          Primary Antibody (green line): Rabbit Anti- CD90 antibody (bs-0778R)
                          Dilution: 1μg /10^6 cells;
                          Isotype Control Antibody (orange line): Rabbit IgG .
                          Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
                          Dilution: 1μg /test.
                          The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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